In Vitro Shoot Multiplication of Garlic (Allium Sativum L.)
Dharshita Patel
Kadi Sarva Vishwavidyalaya Gandhinagar
Rajiv Nayak
Kadi Sarva Vishwavidyalaya Gandhinagar
Ram. S.Suthar
Assistant professor, Department of Biotechnology. P.S.Science & H.D.Patel Arts college, S.V.Campus, Kadi.
Keywords: Shoot apex, Bulb let, Phytohormone, Garlic and GA3.
Abstract
Shoot apex of Garlic, as explants, was used for experiment of bulb formation and shoot multiplication. HgCl2 (0.1%) as surface sterilizing agent was exposed five and ten minutes time duration. GA3 were added to MS basal medium and different concentration of full strength MS medium, half strength MS, one fourth MS and three fourth MS were taken to study their response on explants growth and development. In apex explants, best and early response (76%) for bulb formation and multiplication of shoots were observed in half strength MS medium containing 0.1µM GA3. while in three fourth MS medium without GA3, the bulb formation was delayed and minimum (60%).