Mycelia Growth of Pink Oyster (Pleurotus Djmour) Mushroom in Different Culture Media & Environmental Factors
Iftekhar Ahmad
Department of Food Engineering and Tea Technology, Shahjalal University of Science and Technology, Sylhet
Imran Fuad
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet
Zobada Kanak Khan
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet
Keywords: Pink oyster, Mycelium growth, Potato dextrose agar (PDA), Malt extract agar (MEA), Potato yeast dextrose agar (PYDA), Yeast extract agar (YEA).
Abstract
The study was undertaken to determine the optimum culture media and different other factors viz: optimum culture media composition, pH and temperature for a well-known mushroom- pink oyster (Pleurotus djmour). Four different types of culture media as-Potato dextrose agar (PDA), malt extract agar (MEA), potato yeast dextrose agar (PYDA), and yeast extract agar (YEA) was prepared as different media for pure culture growth. Calculation was conducted on average mycelium growth & duration of complete mycelium growth. The highest mycelia growth rate (0.24 cm) was observed for potato dextrose agar media and the lowest mycelia growth (0.11 cm) was observed in yeast media. The highest mycelia growth rate (0.41 cm) was observed at 250C and the lowest mycelia growth (0.20cm) was observed at 300C temperature. Among the nutrient media compositions the fastest mycelium growth rate was observed in PDA media at ratio of 15:150 (Dextrose: Potato) and Minimum mycelia growth rate was observed in PDA media at ratio of 25:250 (Dextrose: Potato). The pH that gives the highest mycelia growth rate (0.45cm) was observed in the pH 6.5 & the lowest (0.11cm) was observed in the pH 4.5 which statistically similar with pH 9.0.